We offer a number of Protein
Expression Services designed to meet specific goals,
ranging from expression of full-length recombinant
proteins from full-length cDNAs, EST sequence
information and genomic sequences.
Protein expression and purification service package include sub-cloning into appropriate expression vector, sequence confirmation by gene sequencing, plasmid maxi preparation, optimization of protein expression, expression confirmation by SDS-PAGE and Western blotting, and purification of expressed protein to >90% purity.
OUR SERVICES :
Protein Expression Sertvices:
» Codon optimization for
bacterial expression and gene synthesis of DNA insert
» Generation of bacterial expression construct(s) in specified vector
» Transformation into an appropriate host and preparation of glycerol stocks
» Screening studies for the best-expressing protein variant
» Optimization of growth conditions
» Expression assessment by SDS-PAGE/Coomassie or Western blot
Protein Purification Sertvices:
» Affinity Chromatography ( IMAC, GST Tagged Purification, Talon resin, Glutathione Sepharose, Heparin Sepharose, Streptavidin Sepharose, anti-Flag, Protein A, Protein G )
» Ion Exchange Chromatography ( Mono-Q, Q Sepharose Fast Flow, Resource Q, Q Sepharose XL, ANX, DEAE Sepharose, SP Sepharose, CM Sepharose )
» Hydrophobic Interaction Chromatography Phenyl and (CH2)n Sepharose )
» Size Exclusion Chromatography
Endotoxin Removal Service:
We manage endotoxin removal from
the first purification step. Starting with exhaustively
cleaned and validated equipment, we use detergent-free
chromatography methods to lower endotoxin levels.
Important Information :
Kindly download the information brochure to know cost of services, terms and conditions, confidentiality agreement etc.
Enquiry at : firstname.lastname@example.org
Call at : +91 - 08377082003 ( Technical Coordinator )