Flow Cytometry Analysis & Custom Assay Services

A flow cytometry test measures fluorescence intensity produced by fluorescently labeled antibodies specific to proteins on or in cells or ligands that bind to specific cell-associated molecules, such as propidium iodide binding to DNA..

Cell Health & Toxicity Profiling:Flow cytometry cell health and toxicity profiling evaluates drug candidates, natural extracts, and chemical compounds by interrogating cellular viability, death pathways, and metabolic stress at single-cell resolution.

- Apoptosis & Necrosis
- Apoptosis detection - Annexin V/PI staining
- Cell Viability & Proliferation
- Cell Cycle Analysis
- Mitochondrial Membrane Potential
- Intracellular ROS Detection
- Cell cycle analysis: Measuring DNA content with dyes like propidium iodide

Immunophenotyping & Expression Analysis:Immunophenotyping identifies and characterizes specific cell lineages, activation stages, and functional marker expression profiles within heterogeneous biological suspensions.

- Intracellular cytokine staining
- Receptor Occupancy Assessments
- Fluorescent Protein Analysis
- Signal Transduction (Phospho-Flow)
- RNA Flow Cytometry
- Phagocytosis & Oxidative Burst

Bio-Analytical & Quality Control Assays:Bio-analytical and Quality Control (QC) flow cytometry assays serve as critical analytical release and characterization.

- Bacterial & Microbiological Analysis
- Cell cycle analysis: Measuring DNA content
- Absolute Cell Counting

Allele Life Sciences provides a comprehensive Flow Cytometry Full Testing Service to evaluate cellular and signaling phenotypes, cell growth changes, cytokine production, cellular division, and even intracellular signaling events.

Flow cytometry plays a crucial role in healthcare diagnostics, offering rapid, sensitive, and quantitative analysis of cells — which is invaluable in diagnosing and monitoring a variety of diseases.

- Hematological Malignancies (Blood Cancers)
- Immunodeficiency Disorders
- Transplantation & Immune Monitoring
- Autoimmune Diseases
- Infectious Disease
- Prenatal and Neonatal Diagnostics

Flow cytometry offers data on chromosome analysis, cell size and quantity, and biological processes including apoptosis and cell adhesion.

Applications of Flow Cytometry

» Proteomics in early discovery
» Gene expression analysis in cell signaling pathways
» Immunophenotyping
» Disease diagnosis and monitoring

Example Results

What a Flow Cytometry Analysis Result Looks Like

Note: A representative flow cytometry scatter plot showing distinct cell populations. Your delivered results include your actual gated plots and population statistics.