Western blot Analysis

Western Blot is important method in cell and molecular biology. Researchers can distinguish particular proteins from a complicated mixture of proteins isolated from cells by utilizing a western blot.

Services:

» Protein Extraction & Quantification
» SDS-PAGE Gel Electrophoresis
» Transfer to Membrane & Blocking
» Antibody Incubation
» Detection & Analysis

To perform a Western blot, polypropylene gel electrophoresis is used. Protein samples can be separated using SDS-PAGE and then moved to a solid substrate, like a PVDF membrane or nitrocellulose. The protein can be absorbed by the solid support while maintaining its biological activity..

Western blotting is primarily used as a confirmatory test following an initial screening test. It helps verify the presence of disease-specific antibodies or proteins, making it a gold standard in certain diagnostic workflows.

- Infectious Disease Diagnosis
- Autoimmune Diseases
- Cancer Diagnostics and Research
- Genetic Disorders
- Neurological Disorders

Western blot quantification, sometimes referred to as densitometry analysis, is a crucial molecular biology technique for precisely measuring and analyzing protein expression levels. .

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