In vitro
pharmacology generally divides assays
into two major categories to distinguish
between a drug simply sticking to a
target versus actually triggering a
biological response.
-
Cytotoxicity & Proliferation Assay
-
Oxidative Stress Assays
- Apoptosis
Assays
- Cell Migration Assay
-
Metabolic Disorder Assay
-
Neurological Disorder Assay
In vitro, cell
analysis involves studying cellular
functions, viability, proliferation,
toxicity, and molecular interactions
under controlled laboratory conditions.
These assays are widely used in drug
discovery, molecular biology, cancer
research, and toxicology.
- MTT Assay
- Trypan Blue Exclusion Assay
- Lactate Dehydrogenase (LDH) Assay
- ATP-Based Luminescence Assay
Real-time PCR (qPCR) is widely used
for in vitro assays to analyze gene
expression, detect mutations, quantify
nucleic acids, and study cell responses.
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