You start with a crude lysate and end with a purified protein. This 20-hour workshop walks through every chromatography-based purification step in between, hands-on — extraction, quantification, dialysis, and Fast Protein Liquid Chromatography (FPLC).
What You'll Actually Do at the Bench
Protein Extraction
You'll extract protein from cells yourself, learning the lysis and handling steps that determine how much usable protein you actually recover before purification even begins.
Protein Quantification
You'll quantify your protein using the Bradford or Lowry method — the standard techniques for determining protein concentration before and after each purification step.
Buffer and Reagent Preparation
You'll prepare the buffers and reagents that purification depends on, understanding how pH, ionic strength, and composition affect protein stability and binding.
Dialysis and Size Exclusion Chromatography
You'll run dialysis for desalting your protein sample, and work through Size Exclusion Chromatography to separate proteins by molecular size.
Purification by FPLC
You'll finish by purifying your protein using Fast Protein Liquid Chromatography (FPLC) — the workhorse instrument behind most modern protein purification pipelines.
Why Protein Purification Skills Matter
- Pharmaceutical industry — essential for producing therapeutic proteins such as insulin, monoclonal antibodies, and vaccines
- Biotechnology sector — used to manufacture enzymes for diagnostics, food processing, and detergents
- Food industry — purified proteins like whey or soy proteins are used as nutritional supplements
- Cosmetic industry — purified proteins are applied in anti-aging and skincare products
- Research and development labs — rely on protein purification to study protein structure, function, and interactions
High purity and activity of proteins are crucial for safety, efficacy, and regulatory compliance across all of these applications — which is exactly why hands-on purification experience is a core skill for biopharmaceutical R&D, enzymology, and structural biology roles.
Who This Workshop Is For
- Students in biotechnology, biochemistry, or pharmacy wanting real FPLC bench time
- Job seekers targeting biopharmaceutical R&D or enzymology roles
- Researchers who need practical grounding in chromatography-based purification
- Professionals upskilling before a protein-focused project or role
Workshop at a Glance
| Detail | What to Expect |
|---|---|
| Duration | 20 hours, up to 5 days (weekend batches available) |
| Format | Hands-on, bench-based, small group |
| Fee | INR 6,000 per workshop (Indian nationals) |
| You'll leave with | A protein purified by you, from lysate to FPLC-purified sample |
Frequently Asked Questions
How does Protein Purification training advance your career?
Protein purification is a core skill for roles in biopharmaceutical R&D, enzymology, and structural biology. Hands-on training across extraction, dialysis, and FPLC strengthens your candidacy for lab and research roles built around protein work.
What techniques will I use during the workshop?
You'll extract protein from cells, quantify it using the Bradford or Lowry method, prepare buffers and reagents, run dialysis and Size Exclusion Chromatography, and purify your sample using FPLC.
Do I need prior chromatography experience?
No. The workshop starts from protein extraction and quantification before moving into dialysis, size exclusion, and FPLC, so it's built for people developing this skill set from scratch.
How long is the workshop and what does it cost?
The workshop runs 20 hours, spread across up to 5 days with weekend batches available, for INR 6,000 per workshop for Indian nationals.