RT-PCR Workshop

Introduction

These training workshops will give you practical experience in applying cutting-edge technologies for better diagnosis of diseases.

Course Outline

- High Quality RNA Extraction, Qualitative & Quantitative Analysis
- First Strand cDNA Synthesis
- Primer Design For Real Time PCR
- Qualitative RT-PCR Analysis for confirmation of gene expression
- Quantitative Real Time PCR Analysis Gene Expression Data Analysis with
- Standard curve and expression Ct Value
- Data Analysis.

Application

Real-Time PCR  is a molecular biology technique used to amplify and quantify DNA or RNA in real time. It has wide applications in various fields like; diagnosing infectious diseases, detecting genetic disorders, cancer research,gene mutations, measuring gene expression levels.

Food industry:, it is used to detect genetically modified organisms (GMOs) and foodborne pathogens.

Pharmaceutical companies use qPCR for quality control and gene expression studies in drug development.

In environmental monitoring, it detects microbial contamination in water and soil. Its high sensitivity, specificity, and speed make it a powerful tool for both clinical and industrial applications..